Journal: Hippocampus

Article Title: The Suprapyramidal and Infrapyramidal Blades of the Dentate Gyrus Exhibit Different GluN Subunit Content and Dissimilar Frequency‐Dependent Synaptic Plasticity In Vivo

doi: 10.1002/hipo.70002

Figure Lengend Snippet: Expression of subunits of the NMDA receptor is lower in the middle molecular cell layer of the infrapyramidal compared to the suprapyramidal blade of the dentate gyrus. (A) Examination of GluN1 subunits in the middle molecular layer (m.ml) reveals a significantly lower expression in infrapyramidal (iDG) compared to the suprapyramidal (sDG) blade of the dentate gyrus ( N = 19 each). Example of a DAB‐stained image of the hippocampal formation showing the expression of GluN1 subunit of the NMDA receptor. Outlines depict the regions of interest in the m.ml of the sDG and iDG. (B) GluN2A expression is significantly weaker in the m.ml of the iDG compared to the sDG ( N = 20 each). Expression of the GluN2A subunit in the hippocampal formation shown in a representative DAB‐stained image. The regions of interest in the granule cell layer (gcl), outer (o.), inner (i.), and m. ml of sDG and iDG are depicted within the outlines. (C) In the m.ml of the iDG, GluN2B expression is significantly smaller than in the sDG ( N = 20 each). Example of a DAB‐stained photomicrograph showing the expression of the GluN2B subunit in a horizontal section of the hippocampus. The regions of interest in the m.ml are marked by outlines. (A–C) Significant differences are marked with asterisks: ** p < 0.01; **** p < 0.0001. GluN1, GluN2A, and GluN2B subunit expression in gcl, o.ml, and i.ml are shown in Supplementary Figure . Statistical results are summarized in Supplementary Table .

Article Snippet: For this, sections were incubated with primary antibody solutions containing mouse monoclonal anti‐NMDAR1 (GluN1) (1:200; 556308, PharMingen, Becton, Dickinson and Company, Frankline Lakes, USA) or rabbit polyclonal anti‐NMDAε1 (GluN2A) (1:750; sc‐9056, Santa Cruz Biotechnology, Santa Cruz, USA), in tris‐buffered saline containing 0.2% Triton X‐100 and 1% BSA, for 5 days at 4°C.

Techniques: Expressing, Staining

Journal: Scientific Reports

Article Title: AIM2 promotes excitatory glutamate receptor expression by inhibiting STING and contributes to bone cancer pain in male mice

doi: 10.1038/s41598-024-83027-w

Figure Lengend Snippet: Absent in melanoma 2 (AIM2)-Lentivirus (LV) and 5,6-dimethyl-9-oxo-9H-xanthene-4-acetic acid (DMXAA) suppress N-methyl-D-aspartate receptor 1 (GluN1) expression in the spinal dorsal horn of mice with bone cancer pain (BCP). ( A ) Double immunofluorescence staining with anti-AIM2 (green) and anti-GluN1 (red) in the spinal dorsal horn of the wild type (WT) mice (n = 5). ( B ) Immunofluorescence staining with anti-GluN1 (red) in the spinal dorsal horn of the sham, BCP, BCP + CON-LV, and BCP + AIM2-LV. DAPI (blue) was used to stain the nucleus. The precise injection site of the virus in the dorsal horn was indicated by the green fluorescence, which represented the neuron transfected with lentivirus (n = 5). ( C ) Quantitative analysis of GluN1 expression by western blot (WB) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) (* P < 0.05 vs. the sham; # P < 0.05 vs. the BCP + CON-LV; n = 5; mean ± SEM; one-way ANOVA followed by Tukey’s post-hoc test) in the spinal cords of the sham, BCP, BCP + CON-LV, and BCP + AIM2-LV groups on day 7 post-operation. β-Actin and GAPDH were used as loading controls, respectively. ( D ) Quantitative analysis of GluN1 expression by WB and qRT-PCR (* P < 0.05 vs. the sham; # P < 0.05 vs. the BCP + CON-LV; n = 5; mean ± SEM; one-way ANOVA followed by Tukey’s post-hoc test) in the spinal cords of sham, BCP, BCP + vehicle, and BCP + DMXAA groups on day 7 post-operation. β-Actin and GAPDH were used as loading controls, respectively. n represents the number of experimental animals in each group.

Article Snippet: The antibodies used were as follow: mouse anti-AIM2 monoclonal antibody (1:300; cat. no.: 66902-1-IG, RRID: AB_2882230; Proteintech, USA) ; rabbit anti-AIM2 polyclonal antibody (1:300; cat. no.: bs-5986R, RRID: AB_11074508; Bioss, China) ; mouse anti-GluN1 monoclonal antibody (1:300; cat. no.: 32-0500, RRID: AB_86917; Thermo Fisher Scientific, USA) ; rabbit anti-STING polyclonal antibody (1:300; cat. no.: 19851-1-AP, RRID: AB_10665370; Proteintech, USA) ; mouse anti-Synapsin1 monoclonal antibody (1:300; cat. no.: MA5-31919, RRID: AB_2787542; Thermo Fisher Scientific, USA); guinea pig anti-MAP2 monoclonal antibody (1:300; cat. no.: 188004, RRID: AB_2138181; Synaptic Systems, Germany); mouse anti-Iba1 antibody (1:200; cat. no.: sc-32725, RRID: AB_667733; Santa Cruz, USA).

Techniques: Expressing, Double Immunofluorescence Staining, Immunofluorescence, Staining, Injection, Virus, Fluorescence, Transfection, Western Blot, Reverse Transcription, Polymerase Chain Reaction, Quantitative RT-PCR

Journal: Cell

Article Title: Thyroid hormone remodels cortex to coordinate body-wide metabolism and exploration

doi: 10.1016/j.cell.2024.07.041

Figure Lengend Snippet:

Article Snippet: Mouse anti-GluN1 , SySy , Cat# 114011; RRID: AB_887750.

Techniques: Virus, Plasmid Preparation, Recombinant, Control, Protease Inhibitor, RNAscope, Multiplex Assay, Negative Control, Software, Modification